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One Stop Microfluidics Shop

LAB TOOLS

Free calculators for microfluidics.

37+ interactive tools built for researchers. Buffer recipes, dilution volumes, pressure drop, and more.

Assay Analyser — 4PL Curve Fit

Upload plate reader data (ELISA, immunoassay), fit a 4-parameter logistic calibration curve, and interpolate unknown sample concentrations with QC validation.

4PL CurveELISACalibrationQC
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Primer Tm Calculator

Calculate oligonucleotide melting temperature using the nearest-neighbour method. Adjust Na+ and primer concentration for on-chip PCR primer design.

TmPCRNearest-NeighbourOn-Chip
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Sequence GC Content Analyser

Analyse DNA or RNA sequence composition — GC%, base counts, molecular weight and visual base distribution for probe and amplicon design.

GC%DNARNAComposition
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Restriction Enzyme Cut Site Finder

Scan a DNA sequence for restriction enzyme recognition sites. View cut positions, fragment sizes and a linear map for cloning design.

RestrictionCloningCut SitesFragments
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Channel Dimension Calculator

Calculate microfluidic channel dimensions from target shear stress, or find shear stress from known geometry. Includes hydraulic diameter, velocity and Reynolds number.

Shear StressChannel DesignHydraulic DhMicrofluidics
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Residence Time Calculator

Calculate how long a sample spends in each section of a microfluidic chip. Add multiple channel segments — straight, serpentine, tubing, chambers.

Residence TimeVolumeSegmentsOn-Chip
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LOD / LOQ Calculator

Determine limit of detection and limit of quantification from blank replicates or calibration curve data. Essential for validating on-chip assay sensitivity.

LODLOQSensitivityValidation
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Probit LOD95 Calculator

Fit probit regression to binary diagnostic assay data and estimate LOD95 with confidence intervals. For PCR, ELISA, and other yes/no diagnostic dilution series.

ProbitLOD95Binary AssayDiagnostics
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Capillary Pressure Calculator

Calculate capillary pressure in circular or rectangular microchannels using the Young-Laplace equation. Adjust surface tension, contact angle, and channel geometry.

Young-LaplaceSurface TensionContact AngleMicrofluidics
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Droplet Size Estimator

Estimate droplet diameter, volume, and generation frequency for T-junction and flow-focusing geometries. Calculates Capillary number from your flow conditions.

Droplet GenerationT-JunctionFlow-FocusingCa
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Diffusion & Mixing Time Estimator

Estimate diffusion time, Péclet number, and required channel length for complete mixing in laminar microfluidic flow. Includes presets for small molecules, proteins, and cells.

DiffusionPéclet NumberMixing LengthLaminar Flow
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Two-Phase Flow Regime Predictor

Predict the flow regime (squeezing, dripping, jetting) in a microfluidic channel from Capillary and Weber numbers. Includes visual flow pattern diagrams.

Two-PhaseCa NumberWe NumberFlow Regime
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Chip Volume Calculator

Add multiple channel segments to calculate total internal volume of a microfluidic chip. Includes dead volume estimation and reagent planning with waste factor.

Internal VolumeDead VolumeReagent PlanningChip Design
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ELISA Plate Layout Planner

Interactive 96-well plate map for ELISA assay planning. Assign standards, samples, blanks and controls with colour coding. Auto-fill dilution series and export as CSV.

96-WellELISAPlate MapAssay Planning
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Protein Molecular Weight Calculator

Calculate protein molecular weight, extinction coefficient (ε₂₈₀), isoelectric point (pI), and amino acid composition from a sequence. Supports FASTA input.

Protein MWpIε₂₈₀Composition
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Buffer Recipe Calculator

Calculate exact component masses for common lab buffers — PBS, Tris-HCl, HEPES, MES, Citrate, Acetate, MOPS, Bicarbonate — at your target pH, concentration, and volume.

BufferTrisHEPESPBS
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DNA Ligation Calculator

Calculate the amount of insert DNA needed for ligation at a target insert:vector molar ratio. Outputs a complete reaction recipe with volumes.

LigationCloningInsert:VectorMolecular Biology
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Fluorescence Unit Converter

Convert RFU readings to concentration using a linear standard curve. Enter standards, fit the regression, and convert unknowns with R² validation.

RFUStandard CurveFluorescenceRegression
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Haemocytometer & Cell Seeding Calculator

Full haemocytometer workflow — enter corner-square counts, get cells/mL with viability. Then calculate seeding volumes for any well plate format.

HaemocytometerViabilityCell SeedingWell Plates
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Doubling Time Calculator

Calculate cell doubling time from counts or OD readings. Fits exponential growth, extracts growth rate, and predicts future cell numbers with a growth curve chart.

Doubling TimeGrowth RateOD CurveCell Culture
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Aluminium Mould Generator

Upload STL channel features to generate a 4-inch aluminium mould for thermoplastic hot embossing. Configure base thickness, preview in 3D, and export as STEP.

STL to STEPCNC MouldHot Embossing3D Preview
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Sørensen’s Phosphate Buffer Calculator

Calculate exact volumes and weights of monobasic and dibasic sodium phosphate to prepare a buffer at your target pH and concentration.

BufferpH 5.8–8.0Henderson-Hasselbalch
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Lyophilised Oligo Reconstitution Calculator

Work out the exact volume of TE buffer or water to add to a lyophilised oligonucleotide to reach your desired concentration.

OligosPrimersReconstitution
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Beer-Lambert Absorbance Calculator

Convert between absorbance, concentration, and path length using the Beer-Lambert Law. Includes common extinction coefficients for DNA, RNA, proteins, and dyes.

UV-VisA = εclSpectrophotometry
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Molarity Calculator

Enter the molecular weight from your reagent bottle, pick a concentration and volume, and calculate exactly how much powder to weigh out.

MolarityMassSolution prep
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Reynolds Number Calculator

Calculate the Reynolds number for rectangular microfluidic channels. Includes preset fluid properties, flow regime classification, and Dean number for curved channels.

Re numberFlow regimeMicrofluidics
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Serial Dilution Calculator

Generate a complete serial dilution table with transfer volumes, diluent volumes, and concentrations for each step. Perfect for dose-response and calibration curves.

Dilution seriesCalibrationDose-response
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Flow Rate & Velocity Converter

Convert between flow rate and linear velocity for rectangular or circular microfluidic channels. Shows all common units simultaneously.

Flow rateVelocityUnit conversion
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Dead Volume / Tubing Calculator

Calculate the internal volume of tubing and fittings from inner diameter and length. Includes flush time estimates and preset tubing types (PEEK, silicone, FEP).

TubingDead volumeConnections
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Droplet Volume Calculator

Calculate droplet volume, surface area, and surface-to-volume ratio from diameter (or vice versa). Includes droplets-per-microlitre count for emulsion work.

DropletsEmulsionsVolume
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C1V1=C2V2 Dilution Calculator

Calculate the volume of stock solution needed to prepare a dilution, or the final volume from a known aliquot. The classic dilution equation.

DilutionC1V1=C2V2Stock solutions
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Concentration Unit Converter

Convert between M, mM, µM, nM, mg/mL, µg/mL, ng/mL, and %(w/v). Enter any concentration and see all equivalent units instantly.

Unit conversionMolaritymg/mL
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Centrifuge RCF / g-force Calculator

Convert between RPM and relative centrifugal force (×g). Enter your rotor radius and get the exact RCF, or find the RPM needed for a protocol.

RCFRPM×g conversion
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PCR Master Mix Calculator

Calculate reagent volumes for your PCR master mix. Set the number of reactions, adjust concentrations, and get a pipetting table with built-in overage.

PCRMaster mixReagent volumes
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Cell Counting & Seeding Calculator

Convert haemocytometer counts to cells/mL and calculate the volume needed to seed plates, flasks, or microfluidic devices at a target density.

HaemocytometerCell seedingViability
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DNA/RNA Concentration & Purity Calculator

Calculate nucleic acid concentration from A₂₆₀ readings with 260/280 and 260/230 purity ratios. Colour-coded quality indicators flag contamination.

A₂₆₀260/280 ratioDNA / RNA
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Pressure Drop Calculator

Estimate pressure drop across rectangular or circular microfluidic channels using the Hagen-Poiseuille equation. Includes fluid viscosity presets.

PressureHagen-PoiseuilleChannel design
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Mixing Length Calculator

Estimate the channel length required for diffusive mixing in laminar flow. Calculates Péclet number and suggests passive mixer strategies.

Péclet numberDiffusionT-mixer
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