DNA Ligation Calculator

Calculate insert and vector quantities for molecular cloning reactions

DNA Fragment Information

Vector

Vector Size (bp)

Concentration (ng/µL)

Insert

Insert Size (bp)

Concentration (ng/µL)

Insert : Vector Molar Ratio

Vector Amount to Use (ng)

Ligation Reaction Setup

Ligase Buffer (µL)

T4 DNA Ligase (µL)

Total Reaction Volume (µL)

Disclaimer: Molar ratios are calculated from fragment lengths and assume standard double-stranded DNA. Actual ligation efficiency depends on end type (sticky vs blunt), ligase activity, incubation temperature, and DNA purity. Always optimise conditions empirically.