Haemocytometer & Cell Seeding Calculator

Calculate cell counts from haemocytometer data and determine optimal seeding densities

Cell Counting

Cell counts in corner squares (1-4)

Square 1

Square 2

Square 3

Square 4

Dilution factor

e.g., 2 for 1:1 with trypan blue

Volume of one square (µL)

Total sample volume (mL) - optional

Cell Viability (Trypan Blue)

Live cells counted

Dead cells counted

Cell Seeding Calculator

Target cells per well

Target volume per well (µL)

Plate format

Number of wells

Disclaimer: Cell counts are based on manual haemocytometer counting and assume proper sample preparation (dilution, trypan blue staining). Accuracy depends on counting technique, sample homogeneity, and viability stain quality. Always count a minimum of 100 cells for statistical reliability.

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